Cell Separation by Dielectrophoretic Field-flow-fractionation
نویسندگان
چکیده
منابع مشابه
Cell separation by dielectrophoretic field-flow-fractionation.
Dielectrophoretic field-flow-fractionation (DEP-FFF) was applied to several clinically relevant cell separation problems, including the purging of human breast cancer cells from normal T-lymphocytes and from CD34+ hematopoietic stem cells, the separation of the major leukocyte subpopulations, and the enrichment of leukocytes from blood. Cell separations were achieved in a thin chamber equipped ...
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I present a brief review of a cellular separation technique known as dielectrophoretic field-flowfractionation. The technique relies upon suspending different cells at different heights within a laminar flow profile. The differentially suspended cells, thus, travel at different speeds and elute from the flow chamber at different times resulting in successful separation. An alternate fabrication...
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We describe the development and testing of a setup that allows for DEP field-flow fractionation (DEP-FFF) of irreversibly electroporated, reversibly electroporated, and nonelectroporated cells based on their different polarizabilities. We first optimized the channel and electrode dimensions, flow rate, and electric field parameters for efficient DEP-FFF separation of moderately heat-treated CHO...
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Bhave RR (ed.) (1991) Inorganic Membranes. Synthesis, Characteristics and Applications. New York: Van Nostrand Reinhold. Cheryan M (1998) UltraTltration and MicroTltration Handbook. Lancaster, PA: Technomic. Cheryan M and Alvarez J (1995) Membranes in food processing. In: Noble RD and Stern SA (eds) Membrane Separations. Technology, Principles and Applications, p. 415. Amsterdam: Elsevier. Cher...
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Cell Separation and Protein PuriTcation (1996) Oslo, Norway: Dynal. 165 pp. HaK feli U, SchuK tt W, Teller J and ZborowskiM (eds) (1997) ScientiTc and Clinical Applications of Magnetic Carriers. New York: Plenum Press. Olsvik ", Popovic T, Skjerve E et al. (1994) Magnetic separation techniques in diagnostic microbiology. Clinical Microbiology Reviews 7: 43d54. Recktenwald D and Radbruch A (eds)...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2000
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac990922o